KROK 1 2015 – Question 112 · KROK (Medicine) · Biological Chemistry

A doctor was addressed by a 30-yearold man. There is a probability of the patient being HIV-positive. To clarify the diagnosis the doctor proposed to perform polymerase chain reaction. The basic process in this kind of investigation is:

  1. A. Gene amplification
  2. B. Transcription
  3. C. Genetic recombination
  4. D. Chromosome mutation
  5. E. Genomic mutation
Hint

Think about what happens to a chosen DNA fragment over the repeated temperature cycles of PCR, and why that helps to detect a very small amount of viral material.

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