KROK 1 2007 – Question 76 · KROK (Medicine) · Microbiology, Virology, and Immunology

In order to estimate toxigenity of diphtheria agents obtained from patients the cultures were inoculated on Petri dish with nutrient agar on either side of a filter paper strip that was put into the centre and moistened with antidiphtheric antitoxic serum. After incubation of inoculations in agar the strip-like areas of medium turbidity were found between separate cultures and the strip of filter paper. What immunological reaction was conducted?

  1. A. Coomb’s test
  2. B. Rings precipitation reaction
  3. C. Opsonization reaction
  4. D. Agglutination reaction
  5. E. Precipitation gel reaction
Hint

Think about what forms when a soluble toxin diffusing from the colonies meets antibody diffusing from the paper strip, in the proper proportion, inside a semisolid medium.

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