KROK 1 2011 – Question 44 · KROK (Medicine) · Microbiology, Virology, and Immunology

In order to determine toxigenicity of diphtheria bacilli a strip of filter paper impregnated with antitoxic diphtherial serum was put on the dense nutrient medium. There were also inoculated a microbial culture under examination and a strain that is known to be toxigenic. If the microbial culture under examination produces exotoxin, this will result in formation of:

  1. A. Zones of lecithovitellinous activity
  2. B. Haemolysis zones
  3. C. Zones of diffuse opacification
  4. D. Precipitin lines
  5. E. Precipitin ring
Hint

Consider what the toxin and the antitoxin serum do when they diffuse towards each other through agar, and what the visible sign of their meeting looks like.

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