KROK 1 Stomatology 2013 – Question 133 · KROK (Dentistry)
In a bacteriological laboratory some bacterial smears had to be stained by Gram’s method. For this purpose the following reagents were prepared: gentian violet, Lugol’s solution, aqueous fuchsin solution. What other reagent is required?
- A. Carbolic fuchsin
- B. 96% ethanol
- C. 3% hydrogen peroxide
- D. 5% sulfuric acid
- E. Methylene blue solution
Hint
Recall the four steps of Gram staining and which step separates Gram-positive from Gram-negative cells.
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